fu tet o hoct4 backbone (Addgene inc)
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Addgene inc
fu tet o hoct4 backbone
Fu Tet O Hoct4 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fu+tet+o+hoct4+backbone/FU-tet-o-hOct4+(Plasmid+%2319778)/pm37235474-184-46-48
Average 93 stars, based on 18 article reviews
Fu Tet O Hoct4 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fu+tet+o+hoct4+backbone/FU-tet-o-hOct4+(Plasmid+%2319778)/pm37235474-184-46-48
Average 93 stars, based on 18 article reviews
fu tet o hoct4 backbone - by Bioz Stars,
2026-10
93/100 stars
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Mutagenesis:Article Title: Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems. Article Snippet: For the construction of FU-tet-o-GFP, FU-tet-o-mouse Cdk8-2HA, FU-tet-o-mouse Hnf4a, FU-tet-o-mouse Foxa3, FU-tet-o-human CDK8 plasmids, full-length cDNAs encoding GFP, mouse Cdk8, mouse Hnf4a/Foxa3, and human CDK8 were amplified by PCR and subcloned into the FU-tet-o-hOct4 backbone (Addgene Catalog #19778)37 after EcoR I digestion. .. For the construction of FU-tet-ohuman CDK8-DFG, FU-tet-o-human CDK8-15AA, and FU-tet-o-human CDK8-NMG motif mutant plasmids, full-length cDNAs encoding CDK8-DFG (M174F), CDK8-NMG(D173N), and CDK8-15AA (deletion of the first 14 amino acids) were each generated by 2 rounds of PCR using the Gibson assembly method and then subcloned into Generated:Article Title: Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems. Article Snippet: For the construction of FU-tet-o-GFP, FU-tet-o-mouse Cdk8-2HA, FU-tet-o-mouse Hnf4a, FU-tet-o-mouse Foxa3, FU-tet-o-human CDK8 plasmids, full-length cDNAs encoding GFP, mouse Cdk8, mouse Hnf4a/Foxa3, and human CDK8 were amplified by PCR and subcloned into the FU-tet-o-hOct4 backbone (Addgene Catalog #19778)37 after EcoR I digestion. .. For the construction of FU-tet-ohuman CDK8-DFG, FU-tet-o-human CDK8-15AA, and FU-tet-o-human CDK8-NMG motif mutant plasmids, full-length cDNAs encoding CDK8-DFG (M174F), CDK8-NMG(D173N), and CDK8-15AA (deletion of the first 14 amino acids) were each generated by 2 rounds of PCR using the Gibson assembly method and then subcloned into Polymerase Chain Reaction:Article Title: Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems. Article Snippet: For the construction of FU-tet-o-GFP, FU-tet-o-mouse Cdk8-2HA, FU-tet-o-mouse Hnf4a, FU-tet-o-mouse Foxa3, FU-tet-o-human CDK8 plasmids, full-length cDNAs encoding GFP, mouse Cdk8, mouse Hnf4a/Foxa3, and human CDK8 were amplified by PCR and subcloned into the FU-tet-o-hOct4 backbone (Addgene Catalog #19778)37 after EcoR I digestion. .. For the construction of FU-tet-ohuman CDK8-DFG, FU-tet-o-human CDK8-15AA, and FU-tet-o-human CDK8-NMG motif mutant plasmids, full-length cDNAs encoding CDK8-DFG (M174F), CDK8-NMG(D173N), and CDK8-15AA (deletion of the first 14 amino acids) were each generated by 2 rounds of PCR using the Gibson assembly method and then subcloned into Article Title: Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems. Article Snippet: .. For the construction of FU-tet-o-GFP, FU-tet-o-mouse Cdk8-2HA, FU-tet-o-mouse Hnf4a, FU-tet-o-mouse Foxa3, FU-tet-o-human CDK8 plasmids, full-length cDNAs encoding GFP, mouse Cdk8, mouse Hnf4a/Foxa3, and human CDK8 were amplified by PCR and subcloned into the Amplification:Article Title: Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems. Article Snippet: .. For the construction of FU-tet-o-GFP, FU-tet-o-mouse Cdk8-2HA, FU-tet-o-mouse Hnf4a, FU-tet-o-mouse Foxa3, FU-tet-o-human CDK8 plasmids, full-length cDNAs encoding GFP, mouse Cdk8, mouse Hnf4a/Foxa3, and human CDK8 were amplified by PCR and subcloned into the |